Agarose Powder – High Gel Strength Low EEO

Product Overview

The BKMAMLAB Agarose is a high-performance molecular biology grade agarose powder designed for precise and reliable nucleic acid analysis. If you are wondering what is agarose used for, this product is the ideal medium for routine agarose gel electrophoresis, DNA detection, and RNA analysis.

Engineered with high-purity raw materials, BKMAMLAB Agarose ensures excellent solubility and forms a uniform gel matrix without bubbles or cracks. Its high chemical stability prevents unwanted reactions with nucleic acids or buffer reagents, guaranteeing accurate migration and clear DNA banding. Whether you are conducting PCR analysis, molecular biology research, or teaching basic laboratory experiments, this electrophoresis agarose delivers consistent, high-quality results.

Product Specifications

Key Features & Advantages

1. High Gel Strength – Excellent Fracture Resistance

BKMAMLAB Agarose offers a 1% gel strength of >1200 g/cm². This high strength provides appropriate toughness, making the gel easy to handle and highly resistant to breakage during operation.
Tested Results: Gel strength >1200 g/cm², Gelling Temp 35-37°C, Melting Temp 87-89°C.

2. Low Electroendosmosis (EEO) – Precise DNA Analysis

With an EEO value of ≤0.15, this agarose ensures clear DNA bands and accurate migration after DNA agarose gel electrophoresis. It is perfectly suited for precise nucleic acid analysis and high-resolution gel electrophoresis.
Tested Results: Moisture ≤10%, Ash ≤0.5%, EEO ≤0.15, Sulfide ≤0.15%.

3. High Purity & Chemical Stability – TAE/TBE Compatible

Manufactured with strict formulation control, the agarose forms a uniform gel block free of bubbles or cracks. Its high chemical stability means it does not react with nucleic acids or buffer reagents (such as TAE agarose gel or TBE buffers), effectively avoiding interference with electrophoresis results.

4. Batch Consistency – Quality Assurance

Every batch of BKMAMLAB Agarose comes with a Quality Inspection Report (COA). This strict quality control ensures batch-to-batch consistency, making your experimental results more reliable and reproducible.

5. Wide Range of Applications

This versatile electrophoresis agarose is widely used in molecular biology research, drug development and quality control, teaching and basic experiments, as well as industrial production applications.

Purchasing Guide – How to Choose the Right Size

Instructions for Use & Precautions (How to make agarose gel) 

Step 1: Preparation

Prepare reagents and equipment: Agarose powder, electrophoresis buffer (TAE/TBE), nucleic acid dye, DNA Marker/Ladder, samples, gel casting mold, comb, electrophoresis apparatus, and gel imaging system.

Step 2: Gel Preparation (Agarose gel percentage)

Prepare the agarose gel according to the target DNA size. Refer to the table below for recommended concentrations for 1 agarose gel and others:

10 kb: 0.5%-0.8%

1-10 kb: 1%

0.1-1 kb: 1.5%-2%

<100 bp: 2%-3%

Step 3: Heating & Dissolving

Heat in a microwave oven on high for 1 minute, swirl to mix, and repeat until completely transparent (avoid boiling over). For low-melting-point agarose, use a water bath (≤70°C) to prevent thermal degradation.

Step 4: Adding Dye

If pre-staining is required, cool the solution to around 60°C, add the nucleic acid dye according to the instructions, and mix thoroughly.

Step 5: Casting & Solidification

Slowly pour the fully dissolved agarose into the assembled gel casting mold, avoiding bubbles (use a pipette tip to pop any bubbles). Let it stand at room temperature for 20-30 minutes until the gel is fully solidified.

Step 6: Electrophoresis

Proceed with sample loading, electrophoresis, and imaging analysis.

Precautions:

1. Store properly. After opening, avoid contamination to prevent affecting experimental results.
2. In case of accidental contact, take emergency measures and seek medical attention.
3. For your safety and health, please follow laboratory personal protection requirements.

Attention: For scientific research only, strictly prohibited for clinical use.

Versatile Application Scenarios

• Molecular Biology Research: DNA/RNA analysis, PCR agarose gel electrophoresis.
• Drug Development & Quality Control: Nucleic acid testing in pharmaceutical development.
• Teaching & Basic Experiments: Ideal for academic demonstrations and student labs.
• Industrial Production: Large-scale nucleic acid screening and processing.

Frequently Asked Questions (FAQ) 

Q1: What does agarose do in gel electrophoresis?

A: Agarose acts as a molecular sieve. When an electric field is applied, it allows nucleic acids (DNA/RNA) to migrate through the gel matrix at rates inversely proportional to their size, enabling precise separation and analysis.

Q2: What is the gel strength of this agarose?

A: The 1% gel strength is greater than 1200 g/cm², providing excellent toughness and resistance to breakage.

Q3: What is the EEO value of this product?

A: The Electroendosmosis (EEO) value is ≤0.15, ensuring precise migration and clear DNA bands.

Q4: How should I store the agarose powder?

A: Store at room temperature in a sealed, dry environment.

Q5: What is the shelf life?

A: The shelf life is 60 months from the date of manufacture.

Q6: Where to buy agarose powder for gel electrophoresis?

A: You can purchase directly from BKMAMLAB. We offer 10g and 100g sizes, and bulk inquiries are welcome.

Order Now – Enhance Your Electrophoresis Precision

Whether you are performing routine DNA analysis, advanced molecular biology research, or teaching, BKMAMLAB Agarose Powder offers the perfect combination of high gel strength, low EEO, and chemical purity.

Order your BKMAMLAB Agarose today! Bulk inquiries and custom configurations are welcome.