The micro-Transfer Pipette is a kind of precise liquid-fetching device which can be adjusted freely in a certain capacity range, the basic principle is to rely on the device inside the piston to move up and down, the gas piston moving distance is controlled by the control of the screw structure, push the button to drive the rod to move the piston downward, to eliminate the piston cavity gas. After letting go, Transfer Pipette the piston restores the original position under the action of the reset spring, thus completes the liquid absorption process.
The method of using micro-Transfer Pipette
First, the standard operation of the applicable liquid: water, buffer, dilute salt solution and acid and alkali solution.
1 Press to the first gear, vertically into the liquid surface a few millimeters.
2 slow release of the control button, or the liquid into the suction head over the speed will cause the liquid inverted suction fluid inside the suction volume reduced.
3 The liquid when the wall and have a certain angle, first press to the first gear, a little pause after 1s, the remaining liquid after the accumulation, and then press to the second gear to the remaining liquid all pressure out.
II. Removal of viscous or volatile liquids
When removing viscous or volatile liquids, it is easy to lead to larger volume errors. In order to improve the accuracy of liquid transfer, the following methods are recommended:
1 before moving liquid with liquid pre-wet suction head, that is, repeatedly suck the liquid several times to make the suction head wet, suction liquid or discharge liquid when the best stay a few seconds. Especially for the removal of large volume liquids (e.g. 1000~1), Transfer Pipette it is recommended that the suction head be wetted and then removed.
2 The Reverse Phase transfer method: When the liquid is pressed to the second gear, slowly release the control button, hit the liquid when the first gear can be, some of the liquid residue in the suction head.
III. Common error actions
1) When the liquid is sucked, the liquid is tilted and the liquid is not accurate (it should be vertical suction, slow-absorbing and slow-release).
2 The Assembly suction head, Transfer Pipette force too hard, leading to the suction head is difficult to remove (no need to force too hard, select and the liquid filter matching the suction head).
3) A liquid holder with a residual liquid suction head (should be hung on the liquid holder).
4 Remove the small volume sample with a large range of liquid-transfer device (should select the appropriate scope of the liquid).
5 direct to the second gear suction liquid (should be operated according to the above standard method).
6 use acetone or strong corrosive liquid to clean the liquid (should refer to the correct cleaning method operation).