The purpose of the staining solution is to give the sample a solution that combines coloring through chemical or other effects. For example, when we wake up cells, bacteria or certain tissues, we need to observe the shapes, growth status, etc. under optical microscope, etc., and when using optical microscope In this case, if the sample is not dyed, it is difficult for us to observe the cells, even some cell structures (except for cyters or cells with color or cells in color).
The following is some of the physical and chemical information of the two dyed fluids introduced by the readers today. I hope it will be helpful to the experiments.
Gram -staining solution
This chromosomal solution is one of the most common chromosomal fluids in the microbial culture laboratory. It can initially identify bacteria belong to Gram -positive or negative bacteria. For example, when the laboratory is trained for precious cells, each time the finished product is released, it is necessary to take part as a detection sample for testing test. The Gram -dyeing test is an essential part.
Composition
Gram -staining solution is composed of four dyeing solution of Crystal Purple Dyeing, Logo's iodine, 95%ethanol decocent solution, and Fan Red Reproduction solution.
The principle of dyeing is different through the cell wall composition of Gram -positive bacteria and Gram -negative bacteria, which leads to different colors that are dyed in different ability to combine the chromosomal solution to distinguish the Gram -staining positive (G+) or negative. (G-) bacteria. After dyeing, Gram -positive bacteria were stained with blue -purple, while negative bacteria were stained with pink.
Wright-Giemsa Stain
This chromosomal solution is composed of the effective ingredients of the two dyeing fluids of Wright staining and Giemsa staining. These two dye fluids also have a separate purpose
Wright stain solution
Suitable for dyeing solution for samples such as cells, blood, bone marrow coating or tissue slices. One of the most classic and most commonly used dyeing methods in positioning blood cell dyeing analysis can be used to observe the internal structure of the cells and identify various cells and its abnormal changes.
Giemsa Stain Solution
Suitable for dyeing blood and cell coating, bacteria, chromosomal straps, and native animal parasites. This dyeing solution is more dyed in the creature of the simple cells of parasitic insects.
It can be seen that these two dye solution can dye the samples have many types of cellular types, but they also have their own advantages. When the Wright chromosome staining the cell sample, the chromosome of the nucleus contrast the chromaton staining liquid staining the chromatin The situation is more affinity, and it is more conducive to the observation of the nucleus structure, but the color ability of the Ruican chromosome to the cytoplasm of the cells outside the nucleus and the inter -cytoplasm is not as good as that of the Hamsa chromosome. The degree of dyeing of Giemsa dyeing solution is too deep to the nucleus, and the dyeing of cytoplasm is more affinity. Therefore, these two chromosomal fluids are a complementary relationship on the dyeing effect of cells, so they are often used together.
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