ELISA 96 well plate, all black and all white ELISA plate, flat bottom, luminescent plate, Independent sterilization package, TC adherence treatment, sterile elisa plates
Sterile elisa 96 plate, Fluorescence measurement Luminescent plate analysis plate Removable high adsorption TC treatment independent packaging white bottom transparent
Classification: 16 wells, 48 wells and 96 wells
Brand: BKMAM Biotechnology
Material: Polystyrene
Color: white elisa plate, black elisa plate
Packaging: 1 pc/ bag sterile independent packaging
Product use: general suspension culture, bacterial culture, insect breeding, etc.
ELISA PLATE In enzyme-linked immunosorbent assay (Enzyme Linked Immunosorbent Assay, ELISA), the purity, concentration and ratio of antigen, antibody, labeled antibody or antigen involved in immunological reaction; buffer type, concentration and ion Conditions such as strength, pH and reaction temperature and time play a key role.
The solid-phase polystyrene (Polystyrene) surface as a carrier also plays an important role in the adsorption of antigens, antibodies or antigen-antibody complexes.
Antigens, antibodies, and other biomolecules are adsorbed to the carrier surface by a variety of mechanisms, including passive adsorption through hydrophobic bonds, water/ionic bonds, covalent binding through the introduction of other reactive groups such as amino and carbon groups, and surface modification. Hydrophilic bond after modification treatment.
The type of Elisa plates is mainly selected according to the microplate reader. It is divided into detachable and non-detachable. For non-detachable, the slats on a whole board are connected together, then the detachable means that the slats on the board are separated, and the separated slats are separated. There are 12 holes and 8 holes. Generally, detachable enzyme-labeled plates are used more. If you have bought some such plates before, you can just buy some enzyme-labeled slats. Although the microplates made by different manufacturers look similar on the whole, some small details will be different, such as the structure, etc. This is mainly because it needs to be used with different microplate readers. Also consider what your microplate reader will look like when choosing to buy microplates. However, they are generally suitable, and only individual ones will be different. Because the material of the microplate is generally polystyrene (PS), and the chemical stability of polystyrene is poor, it can be dissolved by a variety of organic solvents (such as aromatic hydrocarbons, halogenated hydrocarbons, etc.), and will be corroded by strong acids and alkalis, Not resistant to grease, easy to change color after being exposed to ultraviolet light, so be sure to pay attention to these during use.
High binding ELISA plate
The protein binding capacity of the ELISA plate after surface TC treatment is greatly enhanced, reaching 300-400ng 1gG/cm2, and the molecular weight of the main binding protein is >10kD. The use of this type of ELISA plate can improve the sensitivity, and can relatively reduce the concentration and dosage of the coated protein, but the disadvantage is that it is easy to produce non-specific reactions. After antigen or antibody coating, non-ionic detergents cannot effectively block unbound protein sites, and protein as a blocking agent is required
Medium binding ELISA plate
The ELISA plate is passively bound to white via surface hydrophobic bonds, and is suitable as a solid phase carrier for macromolecular proteins with a molecular weight of 20D, and its protein binding capacity is 200-300 ng 1gG/cm2. Since this type of ELISA plate only binds to macromolecules, it is suitable for use as a solid-phase carrier for unpurified antibodies or antigens, which can reduce potential non-specific cross-reaction. This type of plate can be used as blocking solution with inert protein or non-ionic detergent
Aminase plate
After surface modification, the alcohol label has a positively charged amino group, and its hydrophobic bond is replaced by a water-killing bond. This type of ELISA plate is suitable as a solid-phase carrier for small molecular proteins. Using the appropriate buffer and pH, its surface can bind negatively charged small molecules via ionic bonds. Due to the hydrophilic properties of its surface and its ability to covalently bind through other cross-linking agents, it can be used to immobilize protein molecules dissolved in detergents such as Triton-100 and Tween20. The disadvantage of this type of plate is that due to the reduced hydrophobicity, some protein molecules cannot be bound; in addition, the surface needs to be effectively blocked. Due to the hydrophilic and covalent surface properties, the blocking solution used must be able to interact with non-reactive amino groups and any functional groups in the chosen crosslinker.
Protein Adsorption Determination of ELISA Plates
Blank polystyrene plates for ELISA can be coated with a known concentration of a protein solution. After the coating, the protein concentration of the solution in the well was measured. The amount of protein in the well before coating minus the amount of protein after coating was the amount of protein adsorbed on the ELISA plate. In this way, the adsorption capacity of the ELISA plate can be known.
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