Cryopreservation tube is also called strain preservation tube, magnetic bead preservation tube, and magnetic bead cryopreservation tube. The commonly used methods for preservation of bacteria in microbiology laboratories include milk method, glycerin method, and inclined plane method. The degree of complexity varies, and the effect varies greatly.
cryopreservation tubes use medical polypropylene (PP) as raw materials and are disposable laboratory consumables for storing biological samples. In the gaseous environment of liquid nitrogen, it can withstand low temperatures to minus 187°C. The unique external rotation design avoids the possibility of cross-contamination. The tube cap is equipped with a silicone pad to prevent liquid leakage, and it can ensure the tightness even at low storage temperatures, which ultimately ensures the safety of the samples in the tube. The cap is equipped with a variety of embeddable color codes for easy identification. The white marking area and clear scale on the tube body are convenient for users to mark and calibrate the volume. The centrifugal force can reach 17000g. All cryotubes are sterilized by gamma, without DNase, RNase, and pyrogen.
cryopreservation tube is also called strain preservation tube, magnetic bead preservation tube, and magnetic bead cryopreservation tube. The commonly used methods for preservation of bacteria in microbiology laboratories include milk method, glycerin method, and inclined plane method. The degree of complexity varies, and the effect varies greatly. At present, most laboratories in the country make bacteria preservation tubes by the experimenters themselves, which not only increases the work intensity, but also because of the limitations of various conditions, the bacteria preservation effect is not always satisfactory.
The creation of strain preservation tube makes these extremely easy.
cryopreservation tube is a general term for the commercial name strain preservation tube.
The cryopreservation tube of strains is mainly composed of three parts: preservation solution, preservation tube and small ceramic beads. It is a kind of laboratory preservation container for strain preservation or transfer. Generally, there are 25 small strains in the preservation tube. Porcelain beads are used for the adsorption and preservation of bacteria. During use, the bacteria are inoculated into the preservation tube and placed in an environment of -20°C or -80°C for long-term preservation. (-20℃ can be stored for one year, -80℃ can be stored for two years).
Let's take a look at the steps of using cryotubes
⑴ Pick a fresh culture from the pure culture of bacteria to form a bacterial suspension with a turbidity of about 3-4 Mai's turbidity and inoculate it in a bacterial preservation tube.
⑵ Tighten the preservation tube, turn it up and down 4-5 times to emulsify the bacteria, without shaking.
⑶ Put the preservation tube in the refrigerator for storage (-20℃--70℃)