How to choose the right PCR tubes?
Answer: BKMAM PCR tubes are Suitable for mainstream PCR instruments and real-time PCR instruments in the market（BD, Beckman and Wanshi PCR instruments）.
Comparison of real-time quantitative PCR, digital PCR and traditional PCR:
Overview of digital PCR: measure the proportion of negative replicates to determine the absolute copy number.
Overview of Real-Time PCR: Measurements are made as PCR amplification occurs.
Overview of traditional PCR: measure the amount of accumulated PCR product at the end of the PCR cycle.
Is digital PCR quantitative?
A: Yes, the proportion of negative RCR responses fits the Poisson statistical algorithm.
Is real-time PCR quantitative?
A: Yes, because data is collected during the exponential (logarithmic) phase of PCR, during which the amount of PCR product is proportional to the amount of nucleic acid template.
Is traditional PCR quantitative?
A: No, but comparing the intensity of the amplified band on the gel to a standard of known concentration can give you a "semi-quantitative" result.
Applications of digital PCR: absolute quantification of viral load, absolute quantification of nucleic acid standards, absolute quantification of next-generation sequencing libraries, detection of rare alleles, absolute quantification of gene expression, mixture enrichment and separation
Applications of real-time PCR: gene expression quantification, microarray validation, quality control and assay validation, pathogen detection
, SNP genotyping, copy number variation, microRNA analysis, virus quantification, siRNA/RNAi experiments
Applications of traditional PCR: DNA amplification, sequencing, genotyping, molecular cloning
Conclusion: Advantages of digital PCR: no need to rely on references or standards, desired precision can be achieved by increasing the total number of PCR replicates, high tolerance to inhibitors, ability to analyze complex mixtures
Unlike traditional qPCR, digital PCR responds linearly to the number of copies that emerge and small fold-change differences can be detected.
Advantages of real-time PCR: increased dynamic range of detection, no post-PCR processing required, detection capable of covering changes as low as 2-fold, data acquisition during the exponential phase of PCR, increase in reporter fluorescence signal with the resulting amplicons Proportional in number, cleavage probes provide a permanent record of cleavage of the amplicons
Disadvantages of traditional PCR: poor accuracy, low sensitivity, small dynamic range < 2 logs, low resolution, non-automation, only differential analysis based on specifications, results not represented as numbers, insufficient quantification using ethidium bromide staining, Post-PCR
For more information on PCR strip tubes, please contact us at any time:
Michael Peng
Changde BKMAM Biotechnology Co., Ltd
Address: Building 1, East Of New Material Industrial Zone,
Linli Economic development zone,Changde, 415200 Hunan
Tel:+86-17363688083
Mob:+8613450479355(whatsapp&wechat)
Skype:peng.michael2011
https://hnbikeman.en.alibaba.com
Email:michael@bkmbio.com
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