Pipettes are often used for the transfer of small or trace amounts of liquids in the laboratory. The specifications are different. Pipettes of different specifications use different sizes of tips. The shapes produced by different manufacturers are also slightly different, but the working principle and operation method are basically the same. Consistent. The pipette is a precision instrument. Care must be taken when using and storing it to prevent damage and affect its range.
Two methods of pipetting:
(1) Forward pipetting: press the button of the pipette to the first stop point, gently release the button to the starting point to complete aspiration, lightly press the button to the first stop point to remove the liquid; stop for a while, continue to press Press the button to the second stop, empty the liquid, and release the button to return to the original point. If necessary, continue pipetting after changing the tip.
The forward pipetting method is suitable for lower density solutions.
(2) Reverse pipetting: press the button of the pipette to the second stop point, and gently release the button to the starting point to complete the pipetting. Absorb more than the set volume of liquid, lightly press the button to the first stop, remove the liquid, and discard the excess liquid remaining in the tip with the tip.
Reverse pipetting is suitable for viscous, volatile or bubble-prone solutions.
Notes on use:
(1) When sucking the liquid, the thumb must be released slowly and steadily, and it is not allowed to release suddenly, in order to prevent the solution from being sucked too fast and flushed into the liquid dispenser to corrode the plunger and cause air leakage;
(2) In order to obtain higher precision, the tip needs to absorb the sample solution once in advance, and then formally pipette, because, when sucking serum protein solution or organic solvent, a layer of "liquid film" will remain on the inner wall of the tip, causing discharge The liquid volume is too small to cause errors;
(3) Liquids with high concentration and viscosity will cause errors. In order to eliminate the compensation amount of the error, it can be determined by experiment. The compensation amount can be set by changing the reading of the reading window by adjusting the knob;
(4) The method of weighing the pure water taken with an analytical balance and calculating it can be used to calibrate the liquid dispenser, and 1 mL of distilled water weighs 0.9982 g at 20°C;
(5) It is very inadvisable to tighten the pipette by repeatedly hitting the tip. Long-term operation will loosen the internal parts and damage the pipette;
(6) When the pipette is not equipped with a tip, do not pipette;
(7) When setting the range, please pay attention to rotating to the desired range, the number is clearly displayed in the window, and the set range is within the range of the pipette. Do not turn the button out of the range, otherwise, the mechanical device will be stuck and damaged. pipette. (8) The pipette is strictly prohibited to absorb highly volatile and corrosive liquids (such as concentrated acid, concentrated alkali, organic matter, etc.). (9) It is strictly forbidden to use a pipette to mix the liquid.
(10) Do not use a large-scale pipette to remove small volumes of liquid, so as not to affect the accuracy. At the same time, if you need to pipette a larger amount of liquid outside the range, please use a pipette for operation.
The selection of pipettes should consider the following aspects:
1. High-quality performance
2. Unique volume control system
3. Consistent accuracy and repeatability
4. Reliable and durable
5. Ergonomic design
The realted products produced by BKMAM:
Pipette tips :https://www.bkmbio.com/liquid-handling/pipette-tips/buy-pipette-tips.html
Filter Pipette tips: https://www.bkmbio.com/liquid-handling/pipette-tips/sterile-filter-tips.html
Rack Pipette tips: https://www.bkmbio.com/liquid-handling/pipette-tips/micropipette-tips-200ul.html
Bag Pipette tips:https://www.bkmbio.com/liquid-handling/pipette-tips/p1000-tips.html