Cell culture is a method that simulates the internal environment (sterility, appropriate temperature, pH and certain nutritional conditions, etc.) in vitro to make it survive, grow, reproduce and maintain its main structure and function. Cell culture is also called cell cloning technology. In biology, the formal term is cell culture technology.
Basic conditions of cell culture
1. Suitable cell culture medium
Appropriate cell culture medium is one of the most important conditions for cell growth and proliferation in vitro. The culture medium not only provides cell nutrition and basic materials to promote cell growth and proliferation, but also provides a living environment for cell growth and reproduction.
2. High quality serum
At present, most synthetic media need to be supplemented with serum. Serum is one of the most important components in cell culture medium. It contains a variety of growth factors and other nutrients required for cell growth.
3. Constant cell growth temperature
To maintain the vigorous growth of cultured cells, there must be a constant and appropriate temperature.
4. Suitable gas environment
Gas is one of the essential conditions for the survival of mammalian cell culture. The main gases required are oxygen and carbon dioxide.
5. cell culture temperature
To maintain the vigorous growth of cultured cells, there must be a constant and appropriate temperature. Different kinds of cells have different requirements for culture temperature. The standard temperature of human cell culture is 36.5 ℃± 0.5 ℃. If you deviate from this temperature range, the normal metabolism of cells will be affected or even die.
6. suitable gas environment
Gas is one of the essential conditions for the survival of mammalian cell culture. The main gases required are oxygen and carbon dioxide. Oxygen participates in the tricarboxylic acid cycle, producing energy for cell growth and proliferation and synthesizing various components required for cell growth.
7.Cell culture consumables
BKMAM product consumables for cell culture, like petri dish, plastic cell culture plate, micro pipette, cell spreader, plastic deep well plate, inoculation, cell lifter, micro pipette,plastic pipette tips and etc.
If you want any lab consumables for cell culture technology, welcome to contact me.
Tina
BKMAM biotechnology Co., Ltd
Tel/ wechat: +86 19936789959
Whatsapp: +86 18102756038
Email: tina@bkmbio.com