In the biological laboratory, the most commonly used liquid transfer tools are pipettes and suction heads. Compared with such tools as rubber tip dropper, the pipette has better accuracy and accuracy, and can meet the requirements of different capacities of pipette. In laboratory low flux liquid transfer operations, pipettes can often meet the needs of liquid transfer. There are multi-channel pipettes and electric pipettes at slightly higher flux. However, in ultra high flux large-scale liquid transfer operations, traditional pipettes are not competent. Especially in the COVID-19 epidemic, there are too many samples to be processed. At this time, automated equipment plays a very important role.
Automatic liquid transfer workstation:
The fully automatic liquid transfer workstation is a device that replaces the traditional manual liquid transfer device and is used for automatic liquid transfer. Compared with the traditional liquid transfer method, the workstation has the advantages of being faster, more accurate and more high-throughput, which improves the liquid transfer efficiency and frees the hands. It is usually used in biopharmaceutics, gene sequencing, hospitals, CDC and other fields that need a large number of fluid transfers. At present, the workstations with the highest market share are still imported brands, most commonly Beckman, Tecan and Hamilton. At present, the most commonly used workstations are mainly these three brands, others such as Thermo, Agilent, and some domestic manufacturers.
In the context of the current large-scale outbreak of the epidemic, large-scale nucleic acid detection requires the use of pipette workstations. The advantages of the application workstation in nucleic acid detection, in addition to the above fast and accurate, can effectively ensure the safety of laboratory personnel. Manual processing of nucleic acid samples has a great risk for laboratory personnel, while automatic processing of samples avoids this problem; On the other hand, the pipette workstation can also be combined with other automatic equipment such as nucleic acid extraction workstation to complete a complete set of automatic processes.
The fully automatic liquid transfer workstation usually includes these parts: liquid transfer module, which is used to complete the liquid transfer operation with the suction head; Monitoring module - monitoring the liquid transfer status to ensure the accuracy of liquid transfer; Workbench - container; Software - control the liquid transfer module and monitor the liquid transfer process.
Automatic suction head:
The automatic suction head is specially suitable for workstations. Different from ordinary suction head, ordinary suction head usually has special and general types, while workstation suction head is usually special and suitable for a certain brand and model, so it needs to be selected according to different models. The manufacturer of the liquid transfer workstation usually produces the corresponding matching suction head. In addition, other brands also provide many common brands of automatic suction heads, among which there are many domestic brands, such as Beckman or Tecan, which are relatively cheap.
The core of using the automation system is still to ensure the accuracy of liquid transfer. Because of the absence of manual operation, how to ensure the tightness of each suction head, the consistency of liquid volume in each channel, and minimize errors are the problems that need to be solved by the liquid transfer workstation. Among them, many workstations are equipped with liquid level detection technology to improve the accuracy of liquid transfer. To put it simply, it is necessary to ensure that the height of the suction head in the liquid is at the right position when it is sucking, so as to avoid too high or too low a height, otherwise the accuracy of liquid transfer will be affected. At present, there are some different types of liquid level detection technologies, such as pressure sensing technology for monitoring the change of air pressure, sensing technology for detecting the change of electrical properties of liquids using capacitive sensors, and sensing technology for detecting the time difference between liquids using waves (sound waves, light). In fact, in addition to the liquid transfer workstation, there are many automatic equipment that use the liquid level detection technology.
A type of conductive sucker can be found on the market, and some are also called liquid induction sucker, which is used for liquid level detection in the liquid transfer workstation.
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