Explosion-proof guide for cryovials
Some of our customers have reported problems with cryovials exploding, How do we correctly use and choose cryovials?
Usually, during the experiment, we may use cryovials to freeze the samples, but when using liquid nitrogen for cryopreservation, the explosion of the cryovials often occurs, which will not only cause the loss of experimental samples but may even cause damage to the samples. Injury to experimenters. So how to avoid this from happening?
reason:
If you want to know how to solve this situation, of course, you need to understand why the cryovial will explode.
First of all, cryopreservation tubes cannot be stored directly in the liquid phase of liquid nitrogen. Because the tube body and tube cover of common cryopreservation tubes are of different materials, the rate of thermal expansion and contraction during freezing is also different. If the tube is placed directly into the liquid phase, it may allow liquid nitrogen to flow into the tube.
When resuscitating the sample next time, put the cryovial into a water bath at 37°C, and the liquid nitrogen in the tube quickly vaporizes and expands, but the gas cannot escape from the tube in time, causing the cryovial to explode.
solution:
1: Do not store the cryovial directly in the liquid phase but in the gas phase. Or put it directly in the refrigerator to freeze, remember not to put it directly below the surface of the liquid nitrogen liquid.
2: Use an internal spin cryovial. Of course, even the internal rotation cryotube cannot be directly placed in the liquid phase, but the low-temperature tolerance of the internal rotation cryotube is better than that of the external screw cap, which can reduce the possibility of explosion and is relatively safer. The external spin cryovial is actually designed for mechanical freezing, so it is not suitable for liquid nitrogen storage.
3: So what should I do if I really need liquid phase preservation? In response to this problem, there are actually cryotube sleeves specially designed for this purpose, which can be used to seal the cryopreservation tube and put it into the liquid phase. Of course, it can also be sealed with parafilm, medical tape, etc. This way there will be almost no explosions.
BKMAM Internal/External thread plastic sterile cryotube with PC cryovials tube box for ultra-low temperature in liquid nitrogen environment
1. Low-temperature resistance, high transparency of the tube body, medical grade 2. high-quality PP(tube material) and HDPE(cap material) material
3. Ethylene oxide sterilization, suitable for scientific research such as cells and viruses
4. Strong sealing, threaded design, silicone sealing ring, to ensure sealing.
Thickened tube wall, with writing area, easy to use and convenient for sample management
5. Preferred high-quality raw materials, Dnase, RNase, pyrogens and endotoxins free, stable properties, suitable for storage in various cells, and can also be used for VTM virus sampling.
6. The tube body is transparent, the scale is clear, and the sample is stored accurately and quantitatively.
7. Scientific sealing design, more resistant to low temperature
8. Cryogenic Storage Vials Small size design, capped cryovials designed to withstand ultra-low temperatures -197℃; various capacities(1ml,1.8ml,2ml,3ml,4ml and 5ml); sterile or non-sterile, and autoclavable 125℃; includes internal and external thread cryovials; ideal for sample storage in vapour state liquid nitrogen.
Our cryo tubes are produced under the ISO9001:2015 quality system, our cryo tubes have obtained CE and FDA certification
We are looking for a sole partner in your market,
Please feel free to contact us at any time.
Mob&whatsapp:008613450479355